Follistatin Protein (FST) (Fc Tag)

Catalog No. ABIN7317802

Recombinant Follistatin protein expressed in HEK-293 Cells.

Quick Overview for Follistatin Protein (FST) (Fc Tag) (ABIN7317802)

Target: Follistatin (FST)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Follistatin protein is labelled with Fc Tag.

Purpose: Recombinant Human Follistatin/FST Protein (Fc Tag)(Active)

Sequence: Met 1-Trp 344

Characteristics: A DNA sequence encoding the human FST isoform FST344 (NP_037541.1) (Met 1-Trp 344) was fused with the Fc region of human IgG1 at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to bind human INHBA-his in a functional ELISA.2. Measured by its ability to bind mouse INHBA-his in a functional ELISA.3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 10 ng/ml Recombinant Human ctivin A.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Follistatin (FST)

Alternative Name: Follistatin/FST

Background:

Background: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. follistatin are expressed and subserve local regulatory roles in numerous extragonadal tissues, including brain, adrenal, bone marrow, and placenta but perhaps most notably in anterior pituitary-the classical target tissue for inhibin, the activin-follistatin system may play a key role in early embryogenesis. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone follistatin is a binding protein to activin. Since activin binds to follistatin, it is imperative to determine the nature of the activin/follistatin binding complex.

Synonym: FS

Molecular Weight: 61.7 kDa

NCBI Accession: NP_037541

Pathways: Negative Regulation of Hormone Secretion