GPT2 Protein (His tag)

Catalog No. ABIN7321245

This Recombinant GPT2 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for GPT2 Protein (His tag) (ABIN7321245)

Target: GPT2 (Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2))

Protein Type: Recombinant

Origin: Rat

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This GPT2 protein is labelled with His tag.

Purpose: Recombinant Rat GPT2 Protein (His Tag)

Sequence: Met 1-Ser 522

Characteristics: A DNA sequence encoding the rat Gpt2 (NP_001012057.1) (Met 1-Ser 522) with a C-terminal polyhistidine tag was expressed.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol, 0.5 mM TCEP

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: GPT2 (Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2))

Alternative Name: GPT2

Background:

Background: Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (Gpt), is a pyridoxal enzyme which catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate, and plays a key role in the intermediary metabolism of glucose and amino acids. As a key enzyme for gluconeogenesis, Gpt is a widely-used serum marker for liver injury. Two ALT isoenzymes have been identified, ALT1 and ALT2 (GPT1 and GPT2), which are encoded by separate genes and share significant sequence homology, but differ in their expression patterns. Gpt1 is widely distributed and mainly expressed in intestine, liver, fat tissues, colon, muscle, and heart, in the order of high to low expression level, whereas Gpt2 expression is more restricted, mainly in liver, muscle, brain, and white adipose tissue. It has been reported that hepatic ALT2 protein is approximately four times higher in male rats than in female rats.

Synonym: ALT2,Cc2-5,Gpt2

Molecular Weight: 55 kDa

NCBI Accession: NP_001012057