ENPEP Protein (AA 41-945) (His tag)

Catalog No. ABIN7195941

This Recombinant ENPEP protein is produced in Baculovirus infected Insect Cells.

Quick Overview for ENPEP Protein (AA 41-945) (His tag) (ABIN7195941)

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Protein Characteristics: AA 41-945

Purification tag / Conjugate: This ENPEP protein is labelled with His tag.

Purpose: Recombinant Rat ENPEP/Aminopeptidase A Protein (aa 41-945, His Tag)(Active)

Sequence: Arg41-Pro945

Characteristics: A DNA sequence encoding the mature form of rat ENPEP (P50123-1) (Arg41-Pro945) was expressed,with a polyhistide tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin (Glu-AMC).The specific activity is >1, 500 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Alternative Name: ENPEP/Aminopeptidase A

Background:

Background: ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.

Synonym: ENPEP

Molecular Weight: 106 kDa

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones