GNB2L1 Protein (His tag,MBP tag)

Catalog No. ABIN7317368

Recombinant GNB2L1 protein expressed in Escherichia coli (E. coli).

Quick Overview for GNB2L1 Protein (His tag,MBP tag) (ABIN7317368)

Target: GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 83 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This GNB2L1 protein is labelled with His tag,MBP tag.

Purpose: Recombinant Human RACK1/GNB2L1 Protein (His & MBP Tag)

Sequence: Met 1-Arg 317

Characteristics: A DNA sequence encoding the human GNB2L1 (P63244) (Met 1-Arg 317) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))

Alternative Name: RACK1/GNB2L1

Background:

Background: Calmodulin-like protein 3 (CALML3) is similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinity, to cellular substrates. Calmodulin-like protein 3 (CALML3) is a tumor-sensitive protein specifically expressed in normal epithelial cells but downregulated in tumorigenesis. Downregulation of the protein is an early event in breast cancer development. One of the most pressing questions raised by the discovery of CLP/CALML3 is that of its potential targets. Although it is 85 % identical to human calmodulin, the distinct properties of CLP suggest that it has specific targets or targets that only partially overlap with those of calmodulin. Research has identified the unconventional myosin-10 (Myo10) as a specific target of CALML3. The discovery of Myo10 as a specific target of CALML3 is highly significant and suggests multiple lines of further research such as investigations of the Ca2+ regulation of Myo10 and the role of the loss of CLP in epithelial differentiation, adhesion, and cancer. Cells expressing CALML3 displayed a striking increase in the number and length of myosin-10-positive filopodia and showed increased mobility in a wound healing assay.

Synonym: Gnb2-rs1,H12.3,HLC-7,PIG21,RACK1

Molecular Weight: 78.7 kDa

UniProt: P63244

Pathways: cAMP Metabolic Process, Positive Regulation of Endopeptidase Activity