HMGB3 Protein (His tag)
Quick Overview for HMGB3 Protein (His tag) (ABIN7318584)
Target
See all HMGB3 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This HMGB3 protein is labelled with His tag.
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Purpose
- Recombinant Human HMGB3 Protein (His Tag)
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Sequence
- Met 1-Glu200
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Characteristics
- Recombinant Human High Mobility Group Protein B3 is produced by our Mammalian expression system and the target gene encoding Met1-Glu200 is expressed with a 6His tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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High Mobility Group Box 3 (HMGB3) (AA 1-200) protein (His tag)
custom-made HMGB3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
High Mobility Group Box 3 (HMGB3) (AA 1-180) protein (His tag)HMGB3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
High Mobility Group Box 3 (HMGB3) (AA 1-200) protein (GST tag)HMGB3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
High Mobility Group Box 3 (HMGB3) (AA 14-176) protein (T7 tag,His tag)HMGB3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % ELISA, WB, SDS, IP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- HMGB3 (High Mobility Group Box 3 (HMGB3))
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Alternative Name
- HMGB3
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Background
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Background: High Mobility Group Protein B3 (HMGB3) belongs to the HMGB family. Members of the HMG box subfamily are thought to be have an important role in DNA replication, nucleosome assembly and transcription. HMGB3 binds preferentiallly single-stranded DNA and unwinds double stranded DNA. HMGB3 consists of 200 amino acids and is localized to the cell nucleus. It contains two HMG box DNA-binding domain. HMGB3 binds preferentially single-stranded DNA and unwinds double stranded DNA.
Synonym: High Mobility Group Protein B3, High Mobility Group Protein 2a, HMG-2a, High Mobility Group Protein 4, HMG-4, HMGB3, HMG2A, HMG4
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Molecular Weight
- 24.0 kDa
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UniProt
- O15347
Target
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