HIV-1 gp160 Protein (His tag)

Catalog No. ABIN7196111

Recombinant HIV-1 gp160 protein expressed in HEK-293 Cells.

Quick Overview for HIV-1 gp160 Protein (His tag) (ABIN7196111)

Target: HIV-1 gp160 (Human Immunodeficiency Virus ENV (HIV-1 gp160))

Protein Type: Recombinant

Origin: Human Immunodeficiency Virus (HIV)

Source: HEK-293 Cells

Purity: > 90 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This HIV-1 gp160 protein is labelled with His tag.

Purpose: Recombinant HIV-1 gp160 Protein (gp120 subunit) (group N, strain 06CM-U14296) (His Tag)

Sequence: Trp32-Arg504

Characteristics: A DNA sequence encoding the HIV-1 Envelope glycoprotein gp160 protein (Trp32-Arg504) was expressed with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend centrifuging the vial before opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: HIV-1 gp160 (Human Immunodeficiency Virus ENV (HIV-1 gp160))

Alternative Name: HIV-1 gp160

Target Type: Viral Protein

Background: The HIV-1 gp120 envelope protein, a glycoprotein that is part of the outer layer of the virus, which is an essential component in the multi-tiered viral entry process. It presents itself as viral membrane spikes consisting of 3 Molecules of gp120 linked together and anchored to the membrane by gp41 protein. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell and this is its best-known and most researched role in HIV infection. However, it is becoming increasingly evident that gp12 might also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. Gp120 binding to its receptor CD4 and co-receptor, CXCR4 or CCR5 is required for fusion of viral and cellular membranes. Several mechanisms might be involved in this process of which gp120 binding to the CD4 receptor of T cells is the best known and most important interaction as it facilitates viral entry into the CD4+ cells and their depletion, a hallmark of the HIV infection. Gp120 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. Despite the overall genetic heterogeneity of the gp120 glycoprotein, the conserved CD4 binding site provides an attractive antiviral target. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.

Molecular Weight: 54.4kDa.