ICAM1 Protein (His tag)

Catalog No. ABIN7320278

Product Details

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ICAM1 protein is labelled with His tag.

Purpose: Recombinant Mouse ICAM-1/CD54 Protein (His Tag)(Active)

Sequence: Met 1-Asn 485

Characteristics: A DNA sequence encoding the extracellular domain of mouse ICAM1 (NP_034623.1) (Met 1-Asn 485) was fused with a polyhistidine tag at the C-terminus.

Purity: > 96 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well), approximately > 40% cells will adhere specifically.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM-1/CD54 (ICAM1 Products)

Target Type: Viral Protein

Background:

Background: Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

Synonym: CD54,Icam-1,Ly-47,MALA-2

Molecular Weight: 51.7 kDa

NCBI Accession: NP_034623

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling