ICAM1 Protein (AA 28-480) (His tag)

Catalog No. ABIN7274835

Product Details

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Protein Type: Recombinant

Protein Characteristics: AA 28-480

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ICAM1 protein is labelled with His tag.

Purpose: Human ICAM-1/CD54 Protein

Sequence: Gln28-Glu480

Characteristics: Recombinant Human ICAM-1/CD54 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Glu480.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ICAM-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM-1 (ICAM1 Products)

Synonyms: BB2 Protein, CD54 Protein, P3.58 Protein, Icam-1 Protein, Ly-47 Protein, MALA-2 Protein, ICAM Protein, ICAM-1 Protein, ICAM1 Protein, intercellular adhesion molecule 1 Protein, intercellular adhesion molecule-1 Protein, ICAM1 Protein, Icam1 Protein, ICAM-1 Protein

Background: Intercellular adhesion molecule-1 (ICAM-1, CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis.

Molecular Weight: 50.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling