IL36A/IL1F6 Protein (His tag)

Catalog No. ABIN7317818

This Recombinant IL36A/IL1F6 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL36A/IL1F6 Protein (His tag) (ABIN7317818)

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 99 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This IL36A/IL1F6 protein is labelled with His tag.

Purpose: Recombinant Human IL1F6/IL36A Protein (His Tag)

Sequence: Lys 6-Phe158

Characteristics: A DNA sequence encoding the mature form of human IL1F6 (Q9UHA7) (Lys 6-Phe158) was expressed with a polyhistide tag at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Alternative Name: IL1F6/IL36A

Background:

Background: Interleukin-1 family member 6 (IL-1F6), also known as interleukin 36, alpha (IL36A), is a pro-inflammatory cytokine which plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta, suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line, NCI/ADR-RES, which naturally expresses IL-1Rrp2, all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6, IL-1F8, and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.

Synonym: Interleukin-36 Alpha, FIL1 Epsilon, Interleukin-1 Epsilon, IL-1 Epsilon, Interleukin-1 Family Member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

Molecular Weight: 19.2 kDa

UniProt: Q9UHA7

Pathways: Cancer Immune Checkpoints