LCAT Protein (His tag)
Quick Overview for LCAT Protein (His tag) (ABIN7318716)
Target
See all LCAT ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This LCAT protein is labelled with His tag.
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Purpose
- Recombinant Human LCAT Protein (His Tag)
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Sequence
- Phe25-Glu440
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Characteristics
- Recombinant Human Lecithin-cholesterol acyltransferase is produced by our Mammalian expression system and the target gene encoding Phe25-Glu440 is expressed with a 6His tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-438) protein (His tag)
LCAT Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 1-440) protein (GST tag)LCAT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lecithin-Cholesterol Acyltransferase (LCAT) proteinLCAT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % pure SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of 50 mM Acetate Buffer pH -4.0.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
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Alternative Name
- LCAT
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Background
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Background: Lipase family. The gene encoding this protein is expressed mainly in brain, liver and testes,followed by secreting into plasma and cerebral spinal fluid. The esterification of cholesterol is required for cholesterol transport. LCAT is a central enzyme in the extracellular metabolism of plasma lipoproteins. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) and a cause of fish-eye disease (FED).
Synonym: Phosphatidylcholine-sterol acyltransferase, also named Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase and LACT, is an extracellular cholesterol esterifying enzyme which belongs to the AB hydrolase superfamily.
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Molecular Weight
- 48.1 kDa
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UniProt
- P04180
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Pathways
- Lipid Metabolism
Target
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