KARS Protein (His tag)
Quick Overview for KARS Protein (His tag) (ABIN7318750)
Target
See all KARS ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This KARS protein is labelled with His tag.
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Purpose
- Recombinant Human KARS Protein (His Tag)
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Sequence
- Ala2-Val597
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Characteristics
- Recombinant Human Lysine--tRNA Ligase is produced by our Mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Lysyl-tRNA Synthetase (KARS) (AA 1-597) protein (GST tag)
KARS Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Lysyl-tRNA Synthetase (KARS) (AA 63-597) protein (His tag)KARS Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Lysyl-tRNA Synthetase (KARS) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)KARS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM DTT,20 % glycerol, pH 8.0.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- KARS (Lysyl-tRNA Synthetase (KARS))
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Alternative Name
- KARS
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Background
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Background: Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).
Synonym: Lysine--tRNA Ligase, Lysyl-tRNA Synthetase, LysRS, KARS, KIAA0070
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Molecular Weight
- 69.1 kDa
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UniProt
- Q15046
Target
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