NCK1 Protein (His tag)

Catalog No. ABIN7318390

This Recombinant NCK1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for NCK1 Protein (His tag) (ABIN7318390)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This NCK1 protein is labelled with His tag.

Purpose: Recombinant Human NCK1 Protein (His Tag)

Sequence: Met 1-Ser377

Characteristics: Recombinant Human Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1 is produced by our E.coli expression system and the target gene encoding Met1-Ser377 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 5 % Mannitol, pH 8.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background:

Background: Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.

Synonym: Cytoplasmic Protein NCK1, NCK adaptor Protein 1, Nck-1, SH2/SH3 Adaptor Protein NCK-Alpha, NCK1, NCK

Molecular Weight: 45.0 kDa

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals