Nectin-4 (NECTIN4) (Active) protein (His tag)

Catalog No. ABIN7318810

This Recombinant protein is expressed in Human Cells.

Quick Overview for Nectin-4 (NECTIN4) (Active) protein (His tag) (ABIN7318810)

Target: Nectin-4 (NECTIN4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Human Nectin-4/NECTIN4 Protein (His Tag)(Active)

Sequence: Gly31-Val351

Characteristics: Recombinant Human Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly31-Val351 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 x 104 cells/well are added to recombinant human Nectin-4 coated plates (10 μg/mL with 100 μL/well), approximately 90% will adhere after 1 hour incubation at 37 °C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Nectin-4 (NECTIN4)

Alternative Name: Nectin-4/NECTIN4

Background:

Background: Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.

Synonym: PVRL4,Nectin-4,Ig superfamily receptor LNIR,Poliovirus receptor-related protein 4,PRR4,LNIR

Molecular Weight: 35.3 kDa

UniProt: Q96NY8