NCF2 Protein
Quick Overview for NCF2 Protein (ABIN7317174)
Target
See all NCF2 ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Recombinant Human NCF2/P67phox Protein
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Sequence
- Met 1-Val526
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Characteristics
- A DNA sequence encoding the human NCF2 (AAH01606.1) (Met1-Val526) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Neutrophil Cytosolic Factor 2 (NCF2) (AA 1-525) protein (His tag)
custom-made NCF2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Neutrophil Cytosolic Factor 2 (NCF2) (AA 1-526) protein (His tag)custom-made NCF2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Neutrophil Cytosolic Factor 2 (NCF2) (AA 1-526) protein (GST tag)NCF2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neutrophil Cytosolic Factor 2 (NCF2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)NCF2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 8.0
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))
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Alternative Name
- NCF2/P67phox
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Background
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Background: NCF2, also known as NCF-2 and p67phox, is a subunit of the multi-protein NADPH oxidase complex. NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.
Synonym: NCF-2,NOXA2,P67-PHOX,P67PHOX
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Molecular Weight
- 59.9 kDa
Target
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