OXSR1 Protein (GST tag)

Catalog No. ABIN7197261

This Recombinant OXSR1 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for OXSR1 Protein (GST tag) (ABIN7197261)

Target: OXSR1 (Oxidative-Stress Responsive 1 (OXSR1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 88 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This OXSR1 protein is labelled with GST tag.

Purpose: Recombinant Human OXSR1/OSR1 Protein (GST Tag)

Sequence: Met 1-Ser 527

Characteristics: A DNA sequence encoding the full length of human OXSR1 (NP_005100.1) (Met 1-Ser 527) was fused with the GST tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.5 mM GSH, 0.5 mM PMSF, 0.5 mM EDTA, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: OXSR1 (Oxidative-Stress Responsive 1 (OXSR1))

Alternative Name: OXSR1/OSR1

Background:

Background: Oxidative stress-responsive 1 protein (OXSR1), also known as Serine/threonine-protein kinase OSR1, is a member of the Ser/Thr protein kinase family of proteins. OXSR1 regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. OXSR1 is a 58 kDa protein of 527 amino acids that is widely expressed in mammalian tissues and cell lines. The amino acid (aa) sequence of the predicted OXSR1 protein is 39 % identical to that of human SOK1. Of potential regulators surveyed, endogenous OXSR1 is activated only by osmotic stresses, notably sorbitol and to a lesser extent NaCl. OXSR1 did not increase the activity of coexpressed JNK, nor did it activate three other MAPKs, p38, ERK2, and ERK5. Phosphorylation by OXSR1 modulates the G protein sensitivity of PAK isoforms. The OXSR1 and SPAK are key enzymes in a signalling cascade regulating the activity of Na+/K+/2Cl- co-transporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which recognizes a unique peptide (Arg-Phe-Xaa-Val) motif. The OXSR1 and SPAK kinases specifically recognize their upstream activators and downstream substrates.

Synonym: OSR1

Molecular Weight: 84 kDa

NCBI Accession: NP_005100

Pathways: Tube Formation