PAK3 Protein (PAK3) (His tag)
Quick Overview for PAK3 Protein (PAK3) (His tag) (ABIN7317075)
Target
See all PAK3 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This PAK3 protein is labelled with His tag.
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Purpose
- Recombinant Human PAK3 Protein (His Tag)(Active)
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Sequence
- Met 1-Arg 544
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Characteristics
- A DNA sequence encoding the human PAK3 isoform 2 (O75914-2) (Met 1-Arg 544) was fused with a polyhistidine tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 98 nmol/min/mg using MBP as substrate.
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PAK3 (PAK3) (AA 1-559) protein (His tag)
custom-made PAK3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PAK3 (PAK3) (AA 1-559) protein (His tag)custom-made PAK3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PAK3 (PAK3) (AA 1-90) protein (GST tag)PAK3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- PAK3
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Alternative Name
- PAK3
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Background
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Background: PAK3 is a member of PAK proteins, a family of serine/threonine p21-activating kinases, serve as effectors of small Rho GTPases Cdc42 and RAC and have been implicated in a wide range of biological activities. There are six mammalian PAKs which can be divided into two groups: group I PAKs (PAK1-3) and group II PAKs (PAK4-6). Although the two PAK groups are architecturally similar there are differences in their mode of regulation suggesting their cellular functions are likely to be different. Group I p21-activated kinases (PAK1/2/3) is demonstrated as ERK3/ERK4 activation loop kinases. It has been shown that group I PAKs phosphorylate ERK3 and ERK4 on Ser-189 and Ser-186, respectively, both in vitro and in vivo, and that expression of activated Rac1 augments this response. Besides regulation enzymatic activation of ERK3/ERK4, PAKs can also play roles in downstream activation of MAP kinase-activated protein kinase 5 (MK5) in vivo. Thus, the group I PAKs act as upstream activators of ERK3 and ERK4 and unravel a novel PAK-ERK3/ERK4-MK5 signaling pathway. In clinical, PAK has been proposed as a potential therapeutic target in schwannomas.
Synonym: bPAK;CDKN1A;hPAK3;MRX30;MRX47;OPHN3;PAK3beta
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Molecular Weight
- 62 kDa
Target
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