PAPPA2 Protein (His tag)

Catalog No. ABIN7317837

Recombinant PAPPA2 protein expressed in HEK-293 Cells.

Quick Overview for PAPPA2 Protein (His tag) (ABIN7317837)

Target: PAPPA2 (Pappalysin 2 (PAPPA2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PAPPA2 protein is labelled with His tag.

Purpose: Recombinant Human PAPPA2/Pappalysin 2 Protein (His Tag)

Sequence: Ser 234-Cys 1396

Characteristics: A DNA sequence encoding the human PAPPA2 mature form (NP_064714.2) corresponding to amino acid (Ser 234-Cys 1396) was expressed, with a carboxy-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PAPPA2 (Pappalysin 2 (PAPPA2))

Alternative Name: PAPPA2/Pappalysin 2

Background:

Background: Pappalysin-2/PAPP-A2 is the second member of the pappalysin family of metzincin superfamily, of which PAPP-A is the first member. There is no homology between the prepro-peptides of PAPP-A and PAPP-A2, but 46 % of the residues of mature PAPP-A are also present in mature PAPP-A2. PAPP-A specifically cleaves insulin-like growth factor-binding protein(IGFBP)-4, one of six known modulators of IGF-I and -II, whereas PAPP-A2 specifically cleaved IGFBP-5 at one site, between Ser-143 and Lys-144. In contrast to the cleavage of IGFBP-4 by PAPP-A that strictly requires the presence of IGF, the cleavage of IGFBP-5 by PAPP-A2 was IGF-independent. Recent data firmly establish PAPP-A and IGFBP-4 as an important functional pair in several systems. Because of its close relationship with PAPP-A, both structurally and functionally, PAPP-A2 is a likely candidate for IGFBP-5 proteinase in many tissues and conditioned media where IGFBP-5 proteolysis has been reported.

Synonym: PAPP-A2,PAPP-E,PAPPE,PLAC3

Molecular Weight: 131 kDa

NCBI Accession: NP_064714