PRSS3 Protein (His tag)

Catalog No. ABIN7197546

This Recombinant PRSS3 protein is produced in HEK-293 Cells.

Quick Overview for PRSS3 Protein (His tag) (ABIN7197546)

Target: PRSS3 (Protease, serine, 3 (PRSS3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PRSS3 protein is labelled with His tag.

Purpose: Recombinant Human Trypsin-3/PRSS3 Protein (His Tag)(Active)

Sequence: Met 1-Ser 247

Characteristics: A DNA sequence encoding the human PRSS3 isoform c (P35030-3) (Met 1-Ser 247) was expressed, with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (AnaSpec, Catalog#27114). The specific activity is >4,000 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM MES, 0.6M NaCl, pH 5.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PRSS3 (Protease, serine, 3 (PRSS3))

Alternative Name: Trypsin-3/PRSS3

Background:

Background: Trypsin-3, also known as Trypsin III, brain trypsinogen, Serine protease 3 and PRSS3, is a secreted protein which belongs to the peptidase S1 family. Trypsin-3 / PRSS3 is expressed is in pancreas and brain. It contains one peptidase S1 domain. Trypsin-3 / PRSS3 can degrade intrapancreatic trypsin inhibitors that protect against CP. Genetic variants that cause higher mesotrypsin activity might increase the risk for chronic pancreatitis (CP). A sustained imbalance of pancreatic proteases and their inhibitors seems to be important for the development of CP. The trypsin inhibitor-degrading activity qualified PRSS3 as a candidate for a novel CP susceptibility gene. Trypsin-3 / PRSS3 has been implicated as a putative tumor suppressor gene due to its loss of expression, which is correlated with promoter hypermethylation, in esophageal squamous cell carcinoma and gastric adenocarcinoma.

Synonym: MTG;PRSS4;RP11-176F3.3;T9;TRY3;TRY4

Molecular Weight: 26.6 kDa