PPM1A Protein (His tag)

Catalog No. ABIN7320335

This Recombinant PPM1A protein is expressed in Escherichia coli (E. coli).

Quick Overview for PPM1A Protein (His tag) (ABIN7320335)

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 98 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This PPM1A protein is labelled with His tag.

Purpose: Recombinant Mouse PPM1A Protein (His Tag)

Sequence: Met 1-Trp 382

Characteristics: A DNA sequence encoding the mouse PPM1A (NP_032936.1) (Met 1-Trp 382) was expressed, with a polyhistide tag at the C-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 10 mM HEPES, 500 NaCl, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Alternative Name: PPM1A

Background:

Background: Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.

Synonym: 2310003C21Rik;2900017D14Rik;AI427932;AU017636;MMPa-2;MPPa-1

Molecular Weight: 43.3 kDa

NCBI Accession: NP_032936