SCGB1A1 Protein (His tag)

Catalog No. ABIN7317759

This Recombinant SCGB1A1 protein is produced in HEK-293 Cells.

Quick Overview for SCGB1A1 Protein (His tag) (ABIN7317759)

Target: SCGB1A1 (Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 97 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This SCGB1A1 protein is labelled with His tag.

Purpose: Recombinant Human Uteroglobin/SCGB1A1 Protein (His Tag)(Active)

Sequence: Met 1-Asn 91

Characteristics: A DNA sequence encoding the human SCGB1A1 (P11684) (Met 1-Asn 91) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cell line. When 5 x 10E4cells/well are added to human SCGB1A1 coated plates (2 μg/ml and 100 μl/well), approximately >30% will adhere after one hour at 37 °C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SCGB1A1 (Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1))

Alternative Name: Uteroglobin/SCGB1A1

Background:

Background: Uteroglobin (UG), also known as Secretoglobin 1A member 1 (SCGB1A1), Blastokinin, Clara cell secretor protein (CCSP) or Clara cell-specific 10- kDa protein (CC10), is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is mainly expressed in lung, with anti-inflammatory/immunomodulatory properties. Previous in vitro studies demonstrated that CCAAT/enhancer-binding proteins (C/EBPs) are the major transcription factors for the regulation of SCGB1A1 gene expression, whereas FOXA1 had a minimum effect on the transcription. Uteroglobin is a multifunctional protein with antiinflammatory/immunomodulatory properties. Uteroglobin inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, Uteroglobin manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities. The tissue-specific expression of the Uteroglobin gene is regulated by several steroid hormones, although a nonsteroid hormone, prolactin, further augments its expression in the uterus. Based on its anti-inflammatory and antiallergic properties, Uteroglobin is a potential drug target. The mechanism of Uteroglobin action is likely to be even more complex as it also functions via a putative receptor-mediated pathway.

Synonym: Uteroglobin, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, Secretoglobin family 1A member 1, Urinary protein 1, UP-1, UP1, Urine protein 1, SCGB1A1, CCSP, UGB

Molecular Weight: 9.2 kDa

UniProt: P11684