L-Selectin Protein (His tag)

Catalog No. ABIN7197873

This Recombinant L-Selectin protein is produced in HEK-293 Cells.

Quick Overview for L-Selectin Protein (His tag) (ABIN7197873)

Target: L-Selectin (SELL) (Selectin L (SELL))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 98 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This L-Selectin protein is labelled with His tag.

Purpose: Recombinant Mouse L-Selectin/SELL Protein (His Tag)

Sequence: Met 1-Asn 332

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Asn 332) of mouse SELL (NP_035476.1) precursor was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: L-Selectin/SELL

Background:

Background: L-selectin (SELL), also known as CD62L, is a key adhesion molecule that regulates both the migration of leukocytes at sites of inflammation and the recirculation of lymphocytes between blood and lymphoid tissues. It belongs to the selectin family of proteins, and consisting of a large, highly glycosylated, extracellular domain, a single spanning transmembrane domain and a small cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin-mediated lymphocyte recirculation is required for maintaining the appropriate tissue distribution of lymphocyte subpopulations including naïve and effector subsets such as regulatory T cells. In addition, L-selectin-mediated entry into peripheral lymph nodes is required for optimal induction of lymphocyte homeostatic proliferation during lymphopenia. Importantly, L-selectin has been shown to have both adhesive and signaling functions during leukocyte migration. L-selectin has also been shown to mediate leukocyte recruitment during chronic inflammatory and autoimmune diseases and thus is a potential therapeutic target for drug development.

Synonym: L-selectin;Sell;CD62 antigen-like family member L;Leukocyte adhesion molecule 1;LECAM1;Lymph node homing receptor;Lymphocyte antigen 22;CD62L;LECAM-1;Lnhr;Ly-22;Ly-m22;Lyam-1;Lyam1

Molecular Weight: 34.7 kDa

NCBI Accession: NP_035476