Thrombospondin 1 Protein (THBS1) (His tag)

Catalog No. ABIN7319437

This Recombinant Thrombospondin 1 protein is expressed in Human Cells.

Quick Overview for Thrombospondin 1 Protein (THBS1) (His tag) (ABIN7319437)

Target: Thrombospondin 1 (THBS1)

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 70 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Thrombospondin 1 protein is labelled with His tag.

Purpose: Recombinant Human Thrombospondin-1/THBS1 Protein (His Tag)(Active)

Sequence: Asn19-Pro1170

Characteristics: Recombinant Human Thrombospondin-1 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro1170 is expressed with a 10His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Rhesus macaque CD47-Fc(Cat: PKSQ050076) at 10μg/ml(100 μl/well) can bind Human THBS1-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Thrombospondin 1 (THBS1)

Alternative Name: Thrombospondin-1/THBS1

Background:

Background: Thrombospondin-1 (TSP-1) is a 150-180 kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1domains, 2 EGF-like domains, and 8 TSP type3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules. TSP-1 counteracts the angiogenic, hypotensive, and antithrombotic effects of nitric oxide (NO). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells(EC), and destabilizes adhesive contacts between EC. TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP).

Synonym: Thrombospondin-1, THBS1, TSP, TSP1

Molecular Weight: 129.2 kDa

UniProt: P07996

Pathways: Autophagy