Vitronectin Protein (VTN) (His tag)

Catalog No. ABIN7319175

Recombinant Vitronectin protein expressed in Human Cells.

Quick Overview for Vitronectin Protein (VTN) (His tag) (ABIN7319175)

Target: Vitronectin (VTN)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Vitronectin protein is labelled with His tag.

Purpose: Recombinant Human Vitronectin/VTN Protein (His Tag)(Active)

Sequence: Asp20-Leu478

Characteristics: Recombinant Human Vitronectin is produced by our Mammalian expression system and the target gene encoding Asp20-Leu478 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of Jurkat cells.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Vitronectin (VTN)

Alternative Name: Vitronectin/VTN

Background:

Background: Human Vitronectin/VTN is a cell adhesion and spreading factor. It can be found in the blood and the extracellular matrix (ECM). Vitronectin interacts with glycosaminoglycans and proteoglycans. The multimeric Vitronectin can efficiently bind to and incorporate into the ECM, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Vitronectin can be recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecular. It can as a inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Vitronectin contains an endogenous cleavage site, plus cleavage sites for elastase, thrombin, and plasmin.

Synonym: Vitronectin, VN, S-Protein, Serum-Spreading Factor, V75, VTN

Molecular Weight: 53.4 kDa

UniProt: P04004

Pathways: Autophagy, Smooth Muscle Cell Migration