HCoV-NL63 S1 Protein protein (His tag,ECD)

Catalog No. ABIN7198831

This Recombinant protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for HCoV-NL63 S1 Protein protein (His tag,ECD) (ABIN7198831)

Target: HCoV-NL63 S1 Protein

Protein Type: Recombinant

Origin: Human Coronavirus NL63 (HCoV-NL63)

Source: Baculovirus infected Insect Cells

Purity: > 75 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag,ECD

Purpose: Recombinant HCoV-NL63 S1+S2 Protein (ECD, His Tag)

Sequence: Met1-Pro1296

Characteristics: A DNA sequence encoding the human coronavirus (HCoV-NL63) spike protein (S1+S2 ECD) (APF29071.1) (Met1-Pro1296) was expressed with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM PB, 300 mM NaCl, 10 % glycerol, pH 7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: HCoV-NL63 S1 Protein

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Molecular Weight: 142.4kDa.