HCoV-NL63 Nucleocapsid protein (His tag)

Catalog No. ABIN7198832

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for HCoV-NL63 Nucleocapsid protein (His tag) (ABIN7198832)

Target: HCoV-NL63 Nucleocapsid

Protein Type: Recombinant

Origin: Human Coronavirus NL63 (HCoV-NL63)

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Human coronavirus (HCoV-NL63) Nucleoprotein / NP Protein (His Tag)

Sequence: Met1-His377

Characteristics: A DNA sequence encoding the Human coronavirus (HCoV-NL63) Nucleoprotein (YP_003771.1) (Met1-His377) was expressed with a polyhistide tag at the N-terminus.

Endotoxin Level: Please contact us for more information.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 40 mM PB 500 mM NaCl, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: HCoV-NL63 Nucleocapsid

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Molecular Weight: 43.21kDa.

NCBI Accession: YP_003771