beta-Glucuronidase/Uida/Beta-Gus protein (His tag)

Catalog No. ABIN7320987

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for beta-Glucuronidase/Uida/Beta-Gus protein (His tag) (ABIN7320987)

Target: beta-Glucuronidase/Uida/Beta-Gus

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)

Sequence: Met1-Gln603

Characteristics: Recombinant E.coli Beta-Glucuronidase is produced by our E.coli expression system and the target gene encoding Met1-Gln603 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Comment:

72 kDa

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: beta-Glucuronidase/Uida/Beta-Gus

Background:

Background: Beta-D-glucuronidase from E.coli is a ?highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.

Synonym: Beta-glucuronidase,uidA,GUS

Molecular Weight: 69.9 kDa

UniProt: P05804