AMPK (A2/B1/G1) (Active) Protein

Catalog No. ABIN7317039

This Recombinant protein is produced in Baculovirus infected Insect Cells.

Quick Overview for AMPK (A2/B1/G1) (Active) Protein (ABIN7317039)

Target: AMPK (A2/B1/G1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 94 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human AMPK (G1/B1/A2) Heterotrimer Protein (Active)

Sequence: Met 1-Pro 331

Characteristics: A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1, A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2, A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.

Application Details

Comment:

40, 42 & 95 kDa

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10 % gly, 1 mM GSH, pH 7.4

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: AMPK (A2/B1/G1)

Background: AMPK,AMPKa1

Molecular Weight: 158 (38+30+90) kDa

UniProt: P54619