IgG2a Fc protein (Fc Tag)

Catalog No. ABIN7211747

Recombinant protein expressed in Mammalian Cells.

Quick Overview for IgG2a Fc protein (Fc Tag) (ABIN7211747)

Target: IgG2a Fc

Protein Type: Recombinant

Origin: Mouse

Source: Mammalian Cells

Application: In vivo Studies (in vivo), Isotype Control (IsoC)

Purity: > 95 %

Grade: In vivo Grade

Product Details

Purification tag / Conjugate: Fc Tag

Purpose: In Vivo Grade Recombinant Mouse IgG2a Fc Protein

Characteristics: The in vivo grade recombinant mouse IgG2a Fc protein (mIgG2a Fc, Glu 98 - Lys 330 of the constant region of the IgG2a heavy chain, Accession # CAC20702) was produced in mammalian cells and purified by protein A. The mIgG2a Fc protein includes the CH2 (123-220) and CH3 (236-318) domains of the heavy chain constant region as well as the hinge region (98-122) before the CH2 domain.

Purification: Protein A

Sterility: Sterile filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg)

Application Details

Application Notes: The recombinant mouse IgG2a Fc protein and mutants can be used as the isotype control of the mIgG2a antibodies and the mIgG2a Fc fusion proteins in various in vitro and in vivo experiments.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.

Expiry Date: 3 months

Target Details

Target: IgG2a Fc

Molecular Weight: Predicted 26.4 kDa. Due to glycosylation, the recombinant mouse IgG2a Fc protein migrates to 30-35 kDa based on the Bis-Tris PAGE result.