Alkaline Phosphatase Protein (ALP) (AA 23-506) (His tag)
ALP
Origin: Human
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target See all Alkaline Phosphatase (ALP) products
- Alkaline Phosphatase (ALP)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-506
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Alkaline Phosphatase protein is labelled with His tag.
- Purpose
- Human Alkaline Phosphatase (Placental type) /ALPP Protein
- Sequence
- Ile23-Asp506
- Characteristics
- Recombinant Human Alkaline Phosphatase (Placental type) /ALPP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Asp506.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Alkaline Phosphatase, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 17.1ng/ml determined by ELISA. See testing image for detail.
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Alkaline Phosphatase (ALP) (AA 20-503) protein (His-DYKDDDDK Tag)
ALP Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Alkaline Phosphatase (ALP) (AA 21-501) protein (His tag)ALP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Alkaline Phosphatase (ALP)
- Alternative Name
- Alkaline Phosphatase (ALP Products)
- Background
- Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
- Molecular Weight
- 53.8 kDa. Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result.
- UniProt
- P05187
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