Alkaline Phosphatase Protein (ALP) (AA 20-503) (Biotin,His-Avi Tag)

Catalog No. ABIN7454244

Recombinant Alkaline Phosphatase protein expressed in HEK-293 Cells.

Quick Overview for Alkaline Phosphatase Protein (ALP) (AA 20-503) (Biotin,His-Avi Tag) (ABIN7454244)

Target: Alkaline Phosphatase (ALP)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-503

Purification tag / Conjugate: This Alkaline Phosphatase protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein

Sequence: Ile20-Asp503

Characteristics: Recombinant Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile20-Asp503.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type), His-Avi Tag with the EC50 of 27.8ng/ml determined by ELISA.,Biotinylated Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Alkaline Phosphatase (Germ Type), His-Avi Tag with an affinity constant of 0.04 nM as determined in BLI assay (Gator® Prime).,Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 15000 pmol/min/μg.,The purity of Biotinylated Human Alkaline Phosphatase (Germ type) is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase

Background: Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

Molecular Weight: 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on Bis-Tris PAGE result.

UniProt: P10696