Alkaline Phosphatase Protein (ALP) (AA 20-503) (His tag)

Catalog No. ABIN7454246

Product Details

Target: Alkaline Phosphatase (ALP)

Protein Type: Recombinant

Protein Characteristics: AA 20-503

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Alkaline Phosphatase protein is labelled with His tag.

Purpose: Human Alkaline Phosphatase (Germ type) /ALPG Protein

Sequence: Ile20-Asp503

Characteristics: Recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile20-Asp503.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase (ALP Products)

Synonyms: ALP Protein, ALP1 Protein, CRASH Protein, ECK0378 Protein, JW0374 Protein, psiA Protein, 2410004D18Rik Protein, AU040643 Protein, AW060375 Protein, ILC Protein, CTACK Protein, ESkine Protein, Akp2 Protein, alp Protein, zgc:56672 Protein, DDBDRAFT_0205491 Protein, DDBDRAFT_0231570 Protein, DDB_0205491 Protein, DDB_0231570 Protein, Actn2lp Protein, Alp Protein, asparaginase like 1 Protein, bacterial alkaline phosphatase Protein, C-C motif chemokine ligand 27 Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase Protein, PDZ and LIM domain 3 Protein, ASRGL1 Protein, phoA Protein, Asrgl1 Protein, CCL27 Protein, alpl Protein, alp Protein, Pdlim3 Protein

Background: Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

Molecular Weight: 53.67 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Tris-Bis PAGE result.

UniProt: P10696