APOH Protein (AA 20-345) (His tag)
Quick Overview for APOH Protein (AA 20-345) (His tag) (ABIN7273857)
Target
See all APOH ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 20-345
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Purification tag / Conjugate
- This APOH protein is labelled with His tag.
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Purpose
- Human APOH Protein
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Sequence
- Gly20-Cys345
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Characteristics
- Recombinant Human APOH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys345.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human APOH, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.28μg/ml determined by ELISA. See testing image for detail.
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Apolipoprotein H (Beta-2-Glycoprotein I) (APOH) (AA 20-345) protein (His tag)
APOH Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Apolipoprotein H (Beta-2-Glycoprotein I) (APOH) (AA 20-345) protein (His tag)APOH Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Apolipoprotein H (Beta-2-Glycoprotein I) (APOH) (AA 1-345) protein (GST tag)APOH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Apolipoprotein H (Beta-2-Glycoprotein I) (APOH) (AA 20-345) protein (His tag)APOH Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))
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Alternative Name
- APOH
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Background
- Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele.
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Molecular Weight
- 37.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
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UniProt
- P02749
Target
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