TNFRSF13C Protein (AA 7-71) (His tag)

Catalog No. ABIN7273910

Recombinant TNFRSF13C protein expressed in HEK-293 Cells.

Quick Overview for TNFRSF13C Protein (AA 7-71) (His tag) (ABIN7273910)

Target: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 7-71

Purification tag / Conjugate: This TNFRSF13C protein is labelled with His tag.

Purpose: Human BAFFR/TNFRSF13C Protein

Sequence: Ser7-Ala71

Characteristics: Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser7-Ala71.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human BAFFR, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Alternative Name: BAFFR

Background: BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF.

Molecular Weight: 7.6 kDa. Due to glycosylation, the protein migrates to 13-25 kDa based on Tris-Bis PAGE result.

UniProt: Q96RJ3-1

Pathways: NF-kappaB Signaling