HHLA2 Protein (AA 23-344) (His tag)
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- Target See all HHLA2 Proteins
- HHLA2 (HERV-H LTR-Associating 2 (HHLA2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-344
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HHLA2 protein is labelled with His tag.
- Purpose
- Human B7-H7/HHLA2 Protein
- Sequence
- Ile23-Asn344
- Characteristics
- Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Asn344.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human B7-H7, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 0.20μg/ml determined by ELISA. See testing image for detail.
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HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) protein (Fc Tag)
HHLA2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HERV-H LTR-Associating 2 (HHLA2) (AA 1-414) protein (GST tag)HHLA2 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) (Active) protein (Fc Tag)HHLA2 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by reduced SDS-PAGE. Active
HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) (Active) protein (Fc Tag,AVI tag,Biotin)HHLA2 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- HHLA2 (HERV-H LTR-Associating 2 (HHLA2))
- Alternative Name
- B7-H7 (HHLA2 Products)
- Background
- B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
- Molecular Weight
- 38.1 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
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