Albumin Protein (ALB) (AA 25-608) (His tag)

Catalog No. ABIN7273983

This Recombinant Albumin protein is produced in HEK-293 Cells.

Quick Overview for Albumin Protein (ALB) (AA 25-608) (His tag) (ABIN7273983)

Target: Albumin (ALB)

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-608

Purification tag / Conjugate: This Albumin protein is labelled with His tag.

Purpose: Cynomolgus Serum Albumin Protein

Sequence: Asp25-Ala608

Characteristics: Recombinant Cynomolgus Serum Albumin Protein is expressed from HEK293 with His tag at the C-Terminus., It contains Asp25-Ala608.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.108μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Albumin (ALB)

Alternative Name: Serum Albumin

Background: Albumins are multifunctional proteins present in the blood serum of animals. They can bind and transport a wide variety of ligands which they accommodate due to their conformational flexibility. Serum albumins are highly conserved both in amino acid sequence and three-dimensional structure. Several mammalian and avian serum albumins (SAs) are also allergens. Sensitization to one of the SAs coupled with the high degree of conservation between SAs may result in cross-reactive antibodies in allergic individuals.

Molecular Weight: 67.1 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result.

UniProt: A2V9Z4-1

Pathways: Lipid Metabolism