S100A8/A9 Complex (Calprotectin) Protein (AA 1-93) (His tag)
S100A8/A9
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by Tris-Bis PAGE
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- Target See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) Proteins
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-93
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This S100A8/A9 Complex (Calprotectin) protein is labelled with His tag.
- Purpose
- Human Calprotectin (S100A8&S100A9) Protein
- Sequence
- Met1-Glu93(S100A8) & Thr2-Pro114(S100A9)
- Characteristics
- Recombinant Human Calprotectin (S100A8&S100A9) Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Glu93(S100A8)&Thr2-Pro114(S100A9).
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Calprotectin (S100A8/A9) (AA 1-119) protein (His tag)
S100A8/A9 Origin: Chicken Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 2 mM DTT, PBS ( pH 7.2). Normally 8 % trehalose is added as protectant before lyophilization.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))
- Alternative Name
- Calprotectin (S100A8/A9 Products)
- Background
- Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease.
- Molecular Weight
- 11.7 kDa(S100A8)&13.2 kDa(S100A9) same as Tris-Bis PAGE result.
- Pathways
- S100 Proteins
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