CD161 Protein (AA 67-227) (His-Avi Tag,Biotin)

Catalog No. ABIN7274062

This Recombinant CD161 protein is expressed in HEK-293 Cells.

Quick Overview for CD161 Protein (AA 67-227) (His-Avi Tag,Biotin) (ABIN7274062)

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 67-227

Purification tag / Conjugate: This CD161 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Cynomolgus CD161 Protein

Sequence: Gln67-Leu227

Characteristics: Recombinant Biotinylated Cynomolgus CD161 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln67-Leu227.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Cynomolgus CD161, His Tag at 0.5μg/ml (100μl/Well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Alternative Name: CD161

Background: CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.

Molecular Weight: 21.5 kDa. Due to glycosylation, the protein migrates to 37-42 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005570142