CD14 Protein (CD14) (AA 16-345) (His tag)

Catalog No. ABIN7274087

Product Details

Target: CD14

Protein Type: Recombinant

Protein Characteristics: AA 16-345

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CD14 protein is labelled with His tag.

Purpose: Mouse CD14 Protein

Sequence: Ser16-Pro345

Characteristics: Recombinant Mouse CD14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser16-Pro345.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD14

Alternative Name: CD14 (CD14 Products)

Background: CD14 is a glycosylphosphatidylinositol (GPI)-anchored receptor known to serve as a co-receptor for several Toll-like Receptors (TLRs) both at the cell surface and in the endosomal compartment. CD14 can be expressed by cells of both hematopoietic and non-hematopoietic origin as a cell membrane or secreted protein.

Molecular Weight: 38.19 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_033971

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades