CD20 Protein (AA 141-188) (His-Avi Tag,Biotin)
Quick Overview for CD20 Protein (AA 141-188) (His-Avi Tag,Biotin) (ABIN7274103)
Target
See all CD20 (MS4A1) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 141-188
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Purification tag / Conjugate
- This CD20 protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human CD20/MS4A1 Protein
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Sequence
- Ile141-Ser188
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Characteristics
- Recombinant Biotinylated Human CD20/MS4A1 Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus.It contains Ile141-Ser188.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human CD20, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 87.3ng/ml determined by ELISA. See testing image for detail.
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Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 141-188) protein (His-Avi Tag)
MS4A1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) (Active) protein-VLPMS4A1 Origin: Human Host: HEK-293 Cells VLP > 95 % as determined by HPLC Imm, ELISA, Func, SPR Active
Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) (Active) protein-VLP (Biotin)MS4A1 Origin: Human Host: HEK-293 Cells VLP > 95 % as determined by HPLC Imm, ELISA, Func, SPR Active
Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) protein (His tag)custom-made MS4A1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-291) protein (His tag)custom-made MS4A1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) protein (GST tag)MS4A1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) proteinMS4A1 Origin: Human Host: Mammalian Cells MNP Membrane Nanoparticle
Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) proteinMS4A1 Origin: Human Host: HEK-293 Cells Synthetic Nanodisc
Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) (Active) protein (His tag)MS4A1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) (Active) protein (His tag)MS4A1 Origin: Human Host: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as 0.22μm filtered solution in 20 mM PB, 10 % Glycerol ( pH 7.0).
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Storage
- -80 °C
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Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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Alternative Name
- CD20
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Background
- B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.
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Molecular Weight
- 16.8 kDa same as Tris-Bis PAGE result.
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UniProt
- P11836-1
Target
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