CD3 epsilon Protein (CD3E) (AA 22-48) (Fc-Avi Tag,Biotin)

Catalog No. ABIN7274135

Recombinant CD3 epsilon protein expressed in HEK-293 Cells.

Quick Overview for CD3 epsilon Protein (CD3E) (AA 22-48) (Fc-Avi Tag,Biotin) (ABIN7274135)

Target: CD3 epsilon (CD3E)

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-48

Purification tag / Conjugate: This CD3 epsilon protein is labelled with Fc-Avi Tag,Biotin.

Purpose: Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein

Sequence: Asp22-Thr48

Characteristics: Recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Asp22-Thr48.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CD3 Antibody, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27 peptide, hFc Tag with the EC50 of 6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Molecular Weight: 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: Q95LI5

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway