CD3 epsilon Protein (CD3E) (AA 23-126) (Fc Tag)

Catalog No. ABIN7274147

Recombinant CD3 epsilon protein expressed in HEK-293 Cells.

Quick Overview for CD3 epsilon Protein (CD3E) (AA 23-126) (Fc Tag) (ABIN7274147)

Target: CD3 epsilon (CD3E)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 23-126

Purification tag / Conjugate: This CD3 epsilon protein is labelled with Fc Tag.

Purpose: Human CD3E/CD3 epsilon Protein

Sequence: Asp23-Asp126(C119S, C122S)

Characteristics: Recombinant Human CD3E/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp23-Asp126(C119S, C122S).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 26.7ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Molecular Weight: 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result.

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway