CD28H (AA 23-150) protein (Fc Tag)

Catalog No. ABIN7274253

This Recombinant protein is produced in HEK-293 Cells.

Quick Overview for CD28H (AA 23-150) protein (Fc Tag) (ABIN7274253)

Target: CD28H

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-150

Purification tag / Conjugate: Fc Tag

Purpose: Human CD28H/IGPR-1 Protein

Sequence: Leu23-Gly150

Characteristics: Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu23-Gly150.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human B7-H7, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 81ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD28H

Background: CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.

Molecular Weight: 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

UniProt: Q96BF3-1