CD3 epsilon Protein (CD3E) (AA 22-117) (Fc Tag)

Catalog No. ABIN7274301

This Recombinant CD3 epsilon protein is produced in HEK-293 Cells.

Quick Overview for CD3 epsilon Protein (CD3E) (AA 22-117) (Fc Tag) (ABIN7274301)

Target: CD3 epsilon (CD3E)

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 22-117

Purification tag / Conjugate: This CD3 epsilon protein is labelled with Fc Tag.

Purpose: Cynomolgus CD3E/CD3 epsilon Protein

Sequence: Gln22-Asp117

Characteristics: Recombinant Cynomolgus CD3E/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Molecular Weight: 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.

UniProt: Q95LI5-1

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway