CD3 epsilon Protein (CD3E) (AA 22-117) (Fc Tag)
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- Target See all CD3 epsilon (CD3E) Proteins
- CD3 epsilon (CD3E)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-117
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Origin
- Cynomolgus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD3 epsilon protein is labelled with Fc Tag.
- Purpose
- Cynomolgus CD3E/CD3 epsilon Protein
- Sequence
- Gln22-Asp117
- Characteristics
- Recombinant Cynomolgus CD3E/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.
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CD3 epsilon (CD3E) (AA 22-117) protein (His tag)
CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 23-126) protein (His tag)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) (AA 23-126) protein (Fc Tag)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag)CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag,Biotin)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag,Biotin)CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (Active) protein (mFc Tag)CD3E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD3 epsilon (CD3E) (AA 23-126) protein (His tag,AVI tag,Biotin)CD3E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
CD3 epsilon (CD3E) (AA 22-117) protein (Fc Tag)CD3E Origin: Cynomolgus Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD3 epsilon (CD3E)
- Alternative Name
- CD3E (CD3E Products)
- Background
- CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
- Molecular Weight
- 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
- UniProt
- Q95LI5-1
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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