Adipsin Protein (AA 26-253) (Fc Tag)

Catalog No. ABIN7274335

Product Details

Target: Adipsin (CFD) (Complement Factor D (CFD))

Protein Type: Recombinant

Protein Characteristics: AA 26-253

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Adipsin protein is labelled with Fc Tag.

Purpose: Human Complement Factor D/CFD Protein

Sequence: Ile26-Ala253

Characteristics: Recombinant Human Complement Factor D/CFD Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile26-Ala253.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Complement Factor D, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 42.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Adipsin (CFD) (Complement Factor D (CFD))

Alternative Name: Complement Factor D (CFD Products)

Background: Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.

Molecular Weight: 51.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Pathways: Complement System