Adipsin Protein (AA 21-253) (His tag)
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- Target See all Adipsin (CFD) Proteins
- Adipsin (CFD) (Complement Factor D (CFD))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-253
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Origin
- Rhesus Monkey
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Adipsin protein is labelled with His tag.
- Purpose
- Rhesus macaque Complement Factor D/CFD Protein
- Sequence
- Gln21-Ala253
- Characteristics
- Recombinant Rhesus macaque Complement Factor D/CFD Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Ala253.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Rhesus macaque Complement Factor D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 50 mM Tris, 150 mM NaCl ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Adipsin (CFD) (Complement Factor D (CFD))
- Alternative Name
- Complement Factor D (CFD Products)
- Synonyms
- ADIPSIN Protein, ADN Protein, DF Protein, PFD Protein, Adn Protein, Df Protein, EVE Protein, CFD Protein, adipsin Protein, pfd Protein, cfdl Protein, wu:fb61f12 Protein, zgc:109940 Protein, 10.5 Protein, 10.9 Protein, 14.10 Protein, 20.35 Protein, CG2328 Protein, Dmel\\CG2328 Protein, E(eve) Protein, Eve Protein, F Protein, V Protein, VI Protein, eve2 Protein, even Protein, l(2)46CFg Protein, l(2)46CFh Protein, l(2)46CFj Protein, l(2)46CFp Protein, l(2)46Ce Protein, l(2)46Cg Protein, complement factor D Protein, complement factor D (adipsin) Protein, complement factor D (adipsin) L homeolog Protein, even skipped Protein, CFD Protein, Cfd Protein, cfd.L Protein, cfd Protein, eve Protein
- Background
- Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.
- Molecular Weight
- 25.7 kDa. Due to glycosylation, the protein migrates to 26-30 kDa based on Tris-Bis PAGE result.
- Pathways
- Complement System
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