CLEC4A Protein (AA 70-237) (Fc Tag)
Quick Overview for CLEC4A Protein (AA 70-237) (Fc Tag) (ABIN7274354)
Target
See all CLEC4A ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 70-237
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Purification tag / Conjugate
- This CLEC4A protein is labelled with Fc Tag.
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Purpose
- Human CLEC4A/DCIR Protein
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Sequence
- Gln70-Leu237
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Characteristics
- Recombinant Human CLEC4A/DCIR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln70-Leu237.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human CLEC4A, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC4A Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA. See testing image for detail.
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C-Type Lectin Domain Family 4, Member A (CLEC4A) (AA 1-237) protein (His tag)
custom-made CLEC4A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
C-Type Lectin Domain Family 4, Member A (CLEC4A) (AA 1-238) protein (His tag)custom-made CLEC4A Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
C-Type Lectin Domain Family 4, Member A (CLEC4A) (AA 70-238) protein (Fc Tag)CLEC4A Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
C-Type Lectin Domain Family 4, Member A (CLEC4A) (AA 70-238) protein (His tag)CLEC4A Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
C-Type Lectin Domain Family 4, Member A (CLEC4A) (AA 1-237) protein (GST tag)CLEC4A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CLEC4A (C-Type Lectin Domain Family 4, Member A (CLEC4A))
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Alternative Name
- CLEC4A
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Background
- Clec4a has been reported to be an immune suppressor of dendritic cells (DCs), but its potential role in cancer therapy remains to be elucidated. silencing of Clec4a2 expression via skin delivery of shRNA produces an effective antitumor response and that Clec4a2 shRNA may have therapeutic potential as an adjuvant for cancer immunotherapy.
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Molecular Weight
- 46.7 kDa. Due to glycosylation, the protein migrates to 60-65 kDa based on Tris-Bis PAGE result.
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UniProt
- Q9UMR7-1
Target
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