Clusterin Protein (CLU) (AA 23-449) (His tag)

Catalog No. ABIN7274368

This Recombinant Clusterin protein is expressed in HEK-293 Cells.

Quick Overview for Clusterin Protein (CLU) (AA 23-449) (His tag) (ABIN7274368)

Target: Clusterin (CLU)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 23-449

Purification tag / Conjugate: This Clusterin protein is labelled with His tag.

Purpose: Human Clusterin Protein

Sequence: Asp23-Glu449

Characteristics: Recombinant Human Clusterin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu449.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human Clusterin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Clusterin Antibody, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Clusterin (CLU)

Alternative Name: Clusterin

Background: Clusterin (CLU) is a stress-activated, ATP-independent molecular chaperone, normally secreted from cells, that is up-regulated in Alzheimer disease and in many cancers. It plays important roles in protein homeostasis/proteostasis, inhibition of cell death pathways, and modulation of pro-survival signalling and transcriptional networks. Changes in the CLU gene locus are highly associated with Alzheimer disease, and many therapy-resistant cancers over-express CLU.

Molecular Weight: 51.2 kDa. The protein migrates to 38-41 kDa based on Tris-Bis PAGE result.

UniProt: P10909-1

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling