CSF1R Protein (AA 99-596) (His tag)

Catalog No. ABIN7274422

Product Details

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Protein Type: Recombinant

Protein Characteristics: AA 99-596

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This CSF1R protein is labelled with His tag.

Purpose: Cynomolgus M-CSF R/CSF1R/CD115 Protein

Sequence: Ile99-Pro596

Characteristics: Recombinant Cynomolgus M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile99-Pro596.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus M-CSF R, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. The affinity constant of 0.889 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: M-CSF R (CSF1R Products)

Background: CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Molecular Weight: 56.3 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.

UniProt: G7P8P1

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization