PF4 Protein (AA 32-101) (His-Avi Tag,Biotin)
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- Target See all PF4 Proteins
- PF4 (Platelet Factor 4 (PF4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 32-101
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PF4 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human CXCL4 Protein
- Sequence
- Glu32-Ser101
- Characteristics
- Recombinant Biotinylated Human CXCL4 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Glu32-Ser101.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-CXCL4 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 2.3μg/ml determined by ELISA. See testing image for detail.
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- Discover our top product PF4 Protein
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Platelet Factor 4 (PF4) (AA 32-101) protein (Fc Tag)
PF4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Platelet Factor 4 (PF4) (AA 30-105) protein (Fc Tag)PF4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Platelet Factor 4 (PF4) (AA 32-101) protein (His-Avi Tag)PF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Platelet Factor 4 (PF4) (AA 1-101) protein (GST tag)PF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Platelet Factor 4 (PF4) (AA 34-101) protein (Fc Tag)PF4 Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Platelet Factor 4 (PF4) (AA 32-101) protein (His tag)PF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 4 mM HCl ( pH 3.0).
- Buffer
- Lyophilized from 0.22μm filtered solution in 4 mM HCl ( pH 3.0). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PF4 (Platelet Factor 4 (PF4))
- Alternative Name
- CXCL4 (PF4 Products)
- Background
- Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes.
- Molecular Weight
- 10.68 kDa. The protein migrates to 13 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- NP_002610
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